Figure 3. DAG-based lipoglycans stimulate TLR2-dependant NF-κB activation and IL-8, TNF-α and IL-6 production by human THP-1 monocyte/macrophage cell line.

A) THP-1 cells were incubated with the various stimuli at a concentration ranging from 0.1 to 1000 ng/ml. C, E, G) THP-1 cells were incubated with the various stimuli at a concentration ranging from 0.1 to 10 µg/ml. B, D, F, H) THP-1 cells were pre-incubated or not for 30 min at 37°C with 10 µg/ml of anti-TLR2 or IgG1 isotype control antibodies and stimulated overnight with the different TLR2 ligands. FSL-1, Pam₃CSK₄ FSL-1, MsLM, MlLM, SmLM were tested at a concentration of 1 µg/ml, SaLTA and CgLM at 10 µg/ml. In A and B, NF-κB activation was determined by reading OD at 630 nm. In C to H, cytokines were assayed in the culture supernatant by sandwich ELISA. The results are mean ± SD of triplicate wells and are representative of at least three separate experiments. 10x and 30x mean that the concentration of cytokine detected is amplified 10 and 30 fold respectively. *, P<0.05; **, P<0.01; ***, P<0.001. n.i., not induced.