Figure 4. DAG-based lipoglycans stimulate TLR2-dependant cell surface co-stimulatory molecule CD40 but not MHCII expression by human THP-1 monocyte/macrophage cell line.

THP-1 cells were pre-incubated or not for 30 min at 37°C with 10 µg/ml of anti-TLR2 or IgG1 isotype control antibodies and stimulated overnight with the different TLR2 ligands at a concentration of 1 µg/ml. CD40 (A) and MHC-II (B) expression was monitored by flow cytometry. One representative experiment (from three repeats) is shown. n.i., not induced.