Figure 2. Complementary targets of the MTC-specific IS6110-targeted probe and flanking primers.

Partial alignment of the IS6110 sequence of Mycobacterium tuberculosis complex members with other IS6110-like sequence recently found in M. smegmatis (GenBank access numbers are indicated inside parenthesis). The complementary targets of the P_IS6110 TaqMan probe and flanking primers (FN_IS6110, F_IS6110 and R_IS6110) are indicated (gray boxes). Mismatches in sequences are highlighted in red.