Table 1. Bacterial reference strains and clinical isolates whose cultures were used in the present study for the evaluation of specificity of the amplification assays and respective results.
| Species | Reference strains/Isolates | Presence of IS61101 |
|---|---|---|
| Mycobacterium tuberculosis | ATCC 25177; LNIV 9605 | + |
| M. bovis | AN5; ATCC 27291 (BCG); LNIV 13027; 5530/0/05; 11265; 7230/4; 14421/2; 24497/6; 8855; 5889; 10044; 14577; 13280/6; 13280/4; 34875; and 20564 | + |
| M. caprae | LNIV 17320; 4958/0/05; 8403; 15244; and 20752 | + |
| M. avium subsp. avium | ATCC 25291 | - |
| M. avium subsp. hominissuis | LNIV 23063/4 | - |
| M. avium subsp. paratuberculosis | LNIV 39888 | - |
| M. scrofulaceum | LNIV 31389 | - |
| Acinetobacter baumannii | LNIV 1628/12 | - |
| Arcanobacterium pyogenes | VLA 1884 | - |
| Bacillus licheniformis | VLA 1831 | - |
| Corynebacterium striatum | LNIV 12352 | - |
| Enterobacter amnigenus | LNIV 6050/II | - |
| Klebsiella pneumonia | VLA 1643 | - |
| Listeria monocytogenes | VLA 1774 | - |
| Proteus mirabilis | LNIV 2269/II | - |
| Pseudomonas aeruginosa | VLA 67 | - |
| Salmonella Dublin | VLA 1272 | - |
| Staphylococcus aureus | VLA 1032 | - |
| Streptococcus agalactiae | VLA 33 | - |
| Yersinia enterocolitica | VLA 1884 | - |
ATCC, American Type Culture Collection, USA; LNIV, Laboratório Nacional de Investigação Veterinária (currently INIAV, IP), Lisbon, Portugal; VLA, Veterinary Laboratory Agency, UK; 1Amplification (+) or no amplification (-) of IS6110 element using the real-time PCR assay with P_IS6110 TaqMan probe and respective flanking primers F_IS6110 and R_IS6110.