Table 3. Sequences of primers and probes used in this study.
| Primer/Probe | Sequence (5´-3´) | Complementary target |
|---|---|---|
| F_Actin | GGC TCY ATY CTG GCC TC | β-actin gene of mammals |
| R_Actin | GCA YTT GCG GTG SAC RAT G | |
| P_Actin1 | Cy5.5-TAC TCC TGC TTG CTG ATC CAC ATC-BHQ2 | |
| F_IS6110 | GGG TCG CTT CCA CGA TG | IS6110 element of MTC species |
| FN_IS6110 | CTC GTC CAG CGC CGC TTC GG | |
| R_IS6110 | GGG TCC AGA TGG CTT GC | |
| P_IS6110 2 | FAM-CGC GTC GAG GAC CAT GGA GGT-BHQ1 |
1Probe labeled with Cy5.5 fluorophore and BHQ-2 quencher; 2Probe labeled with carboxyfluorescein (FAM) fluorophore and BHQ-1 quencher