Figure 5. Res inhibits tumor growth in vivo in a Sirt1-dependent manner.

(A) Nude mice were randomly divided into four groups and there were 8 mice for each group. BGC-823 cells (1×10⁶) with different treatments were injected subcutaneously into each group. After implantation, the tumor size was measured weekly. The tumor volumes were calculated by the formula (length) × (width²)/2. (B) The proliferating cells in the tumor specimens from the different groups were detected by Ki67 immunochemistry. Senescence was indicated by β-Gal staining. The representative graphs are shown. Magnification: × 400, bar for 20 µm. (C) Total protein from the tumor specimens was extracted, and the expression of the regulators of cell cycle were detected by western blot. ‘C’ represents control, ‘R’ represents resveratrol, ‘Ci’ represents the stable control lentiviral-shRNA BGC-823 cells, and ‘Si’ represents the stable Sirt1 lentiviral-shRNA BGC-823 cells.