Figure 7. Identification of modifying genes with undetermined roles in dNf1 suppression.

(A) Validation of NAAT1, HERC2 and Hs3st-B as dNf1 modifiers. All three genes were identified by systematic RNAi screening of genes uncovered by suppressing deficiencies. (B) Loss-of-function alleles of Class C Vacuolar Protein Sorting complex subunits carnation (car/Vps33A) and deep-orange (dor/Vps18) increase dNf1 pupal size. C) RNAi-mediated neuronal car or dor knockdown was not particularly effective, suggesting these genes may function elsewhere to modify dNf1-dependent growth.