Figure 9. Several dNf1 pupal size defect suppressors also suppress a NMJ synaptic overgrowth phenotype.

(A–E) Representative micrographs of larval muscle 6/7 NMJs of the indicated genotypes. F: Mean bouton number per NMJ normalized to wild-type control. Compared to wild-type (w¹¹¹⁸; A), dNf1 mutants (dNf1^E2; B) have an increased bouton number. While a cnk loss-of-function allele had no obvious NMJ phenotype, it dominantly suppressed the dNf1 NMJ defect (C). Similarly, the dNf1 NMJ phenotype was suppressed in Df(1)Exel9051 males that lack CCKLR-17D1 (D), while females heterozygous for CCKLR-17D1 (E) showed a lower level of suppression. Spitz (spi) is uncovered by a modifying deficiency but does not affect dNf1 size and was used as a negative control. In panels A–E, scale bars represent 5 µm. In panel F, error bars denote standard error of the mean.