Table 3. Restoration of systemic growth by dNf1 and cAMP/PKA involves different tissues.

Gal4	UAS-dNf1	dnc v107967	2×UAS-PKA*	3×UAS-PKA*	4×UAS-PKA*	5×UAS-PKA*
Act5C	Rescue	Rescue (pupal †)	SV	†	†	†
elav	Rescue	NR	NR	NR	NR	†
elav+Dcr-2	Rescue	NR	n/a	n/a	n/a	n/a
Ras2(41)	Rescue	NR	NR	NR	†	†
Ras2(41)+Dcr-2	Rescue	NR	n/a	n/a	n/a	n/a
C23	Rescue	NR	NR	NR	NR (pupal †)	†
Feb36	NR	Rescue	NR	Rescue	†	†
Aug21	NR	Rescue	Rescue	Rescue	Rescue	†
Akh	NR	Rescue	Rescue	Rescue	Rescue	Rescue (SV)

Act5C-Gal4 driven ubiquitous dNf1 re-expression, or elav-Gal4 and Ras2-Gal4 driven neuronal re-expression rescues the dNf1 pupal size defect, whereas dnc RNAi or UAS-PKA* expression controlled by the same drivers is ineffective. By contrast, expressing dNf1 in specific parts of the neuroendocrine ring gland with the Akh-Gal4, Feb36-Gal4 or Aug21-Gal4 drivers fails to rescue, whereas using the same drivers to express dnc RNAi or attenuated UAS-PKA* transgenes does increase dNf1 pupal size. All crosses produced viable adults unless otherwise indicated.

^†denotes lethality, SV sub-viable, n/a not applicable, NR non-rescue.

The data shown summarize results of a larger effort to identify the tissues in which dNf1 and cAMP/PKA affect systemic growth. Full results are shown in Table S5.