Table 2. Results of the ITC experiments performed with Apo-YLOV.

ligand	binding active Apo-YLOV	∆H (kcal / mol)	T∆S (kcal / mol)	KD (nM)	∆G (kcal / mol)
FMN	96.2%	-24.52 [-24.88; -24.16]	-16.14	715 [645; 791]	-8.38
RF	94.7%	-22.25 [-23.23; -22.46]	-13.45	351 [292; 397]	-8.80
FAD	98.3%	-16.57 [-16.86; -16.30]	-7.50	224 [192; 260]	-9.07

Values in brackets display the upper and lower limits within a 95% confidence interval.