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. 2013 Nov 21;8(11):e79870. doi: 10.1371/journal.pone.0079870

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© 2013 Arezes et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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mRNA levels (A; qRT-PCR) and corresponding NTBI uptake (B) by CD4⁺ and CD8⁺ T lymphocytes following nucleofection with siRNAs specific for ZIP14, DMT1-IRE and DMT1-nIRE, with scrambled siRNAs (siNeg) or with no DNA as controls. Each column represents the mean value (n = 3) ± SD. Statistical significance between samples (grey columns) and controls (white columns) is indicated by * symbols (*p<0.01). No differences in Fe∶citrate uptake were observed after silencing DMT1 or ZIP14.