Figure 8. Involvement of clathrin-mediated endocytosis in NTBI uptake by PBMCs and HepG2 cells.

(A–B) ⁵⁵Fe uptake by PBMCs and HepG2 cells in the presence of 0.45M of sucrose (A) or 80 µM of Dynasore (B) to inhibit clathrin-mediated endocytosis, after incubation with 5 µM of ⁵⁵Fe-citrate (5∶100) at 37°C (C–D) ¹²⁵I-Transferrin internalization in the presence of 0.45M of sucrose (C) or 80 µM of Dynasore (D). Each bar represents a mean value (n = 3) ± SD. Statistical significance is indicated by * symbols (*p<0.01). The inhibition of these pathways did not prevent the uptake of NTBI in both PBMCs and HepG₂ cells but transferrin internalization was decreased.