Table 1. Protein Samples, Plug Maker Parameters and Crystallization Results.
|
Plug Maker Flow Rate (μL/minute)
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
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Sample #
|
Protein
|
Buffer
|
Precipitate
|
Carrier Fluid
|
Crystals?
|
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| Protein | μg | 1g | Start | Finish | Start | Finish | Start | Finish | Start | Finish | μg | 1g |
| Lipase B | 989 | 990 | 2.0 | 2.0 | 0.5 | 1.5 | 2.0 | 1.0 | 5.0 | 5.0 | N | N |
| 991 | 992 | 3.0 | 1.0 | 0.5 | 0.5 | 1.0 | 3.0 | 5.0 | 5.0 | N | N | |
| 1015 | 1016 | 2.0 | 2.0 | 0.5 | 0.5 | 2.0 | 2.0 | 0.1 | 0.1 | N | N | |
| Xylanase | 993 | 994 | 2.0 | 2.0 | 0.5 | 0.5 | 2.0 | 1.0 | 6.5 | 5.5 | Y | N |
| 995 | 996 | 3.0 | 1.0 | 0.5 | 0.5 | 1.0 | 3.0 | 5.5 | 5.5 | N | N | |
| 997 | 998 | 2.0 | 2.0 | 0.5 | 0.5 | 2.0 | 2.0 | 8.0 | 1.5 | N | N | |
| 1027 | 1028 | 2.0 | 2.0 | 0.5 | 0.5 | 2.0 | 2.0 | 0.1 | 0.0 | N | N | |
| 1029 | 1030 | 2.0 | 1.0 | 0.5 | 1.5 | 2.0 | 2.0 | 5.5 | 5.5 | N | N | |
| Lysozyme | 999 | 1000 | 2.0 | 2.0 | 0.5 | 1.5 | 2.0 | 1.0 | 5.5 | 5.5 | Y | Y |
| 1001 | 1002 | 3.0 | 1.0 | 0.5 | 0.5 | 1.0 | 3.0 | 5.5 | 5.5 | Y | Y | |
| 1003 | 1004 | 2.0 | 2.0 | 0.5 | 0.5 | 2.0 | 2.0 | 8.0 | 1.5 | Y | Y | |
| 1005 | 1006 | 2.0 | 2.0 | 0.5 | 0.5 | 2.0 | 2.0 | 0.5 | 0.5 | Y | Y | |
| 1031 | 1032 | 2.0 | 1.0 | 0.5 | 1.5 | 2.0 | 2.0 | 5.5 | 5.5 | Y | Y | |
| 1033 | 1034 | 2.0 | 2.0 | 0.1 | 2.0 | 2.0 | 0.1 | 0.1 | 0.0 | Y | Y | |
| apo-hPPAR-γ LBD | 1017 | 1018 | 1.0 | 3.0 | 0.5 | 0.5 | 3.0 | 1.0 | 8.0 | 8.0 | Y | N |
| 1019 | 1020 | 1.0 | 2.0 | 1.5 | 0.5 | 2.0 | 2.0 | 8.0 | 8.0 | Y | N | |
| 1021 | 1022 | 2.0 | 2.0 | 0.5 | 1.5 | 2.0 | 1.0 | 8.0 | 8.0 | Y | N | |
| 1023 | 1024 | 2.0 | 2.0 | 0.5 | 0.5 | 2.0 | 2.0 | 0.1 | 0.0 | N | N | |
| 1025 | 1026 | 1.0 | 3.0 | 0.5 | 0.5 | 3.0 | 1.0 | 8.0 | 8.0 | Y | N | |
| 1035 | 1036 | 2.0 | 2.0 | 0.1 | 2.0 | 2.0 | 0.1 | 0.1 | 0.0 | Y | Y | |
| 1037 | 1038 | 5.0 | 0.1 | 0.5 | 0.5 | 0.1 | 5.0 | 0.1 | 0.0 | N | Y | |
| Thermolysin | 1007 | 1008 | 2.0 | 2.0 | 0.5 | 1.5 | 2.0 | 1.0 | 5.5 | 5.5 | Y | Y |
| 1009 | 1010 | 1.0 | 3.0 | 0.5 | 0.5 | 3.0 | 1.0 | 5.5 | 5.5 | Y | Y | |
| 1011 | 1012 | 2.0 | 2.0 | 0.5 | 0.5 | 2.0 | 2.0 | 8.0 | 1.5 | Y | Y | |
| 1013 | 1014 | 2.0 | 2.0 | 0.5 | 0.5 | 2.0 | 2.0 | 0.1 | 0.1 | Y | Y | |