Skip to main content
. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Methods. 2013 Jun 26;64(2):10.1016/j.ymeth.2013.06.018. doi: 10.1016/j.ymeth.2013.06.018

Figure 3.

Figure 3

Amplification of the VH and VL regions from single B cells. Subsequent to cell lysis and reverse transcription to yield cDNA, two rounds of PCR are employed to amplify the VH and VL regions. The PCR products are run on an agarose gel and visualized by ethidium bromide staining (~400 bp, dark arrow). (A) Lanes H1–H6 and (B) L1’-L3’ show gene amplification for VH and VL products, respectively, from 6 single B cells. Sequential amplification of VH and then the VL is performed to conserve reagents.