Figure 4. Astrocytes and pericytes phenotype in NHIR.

P1 rat pups were injected with low doses of streptozotocin (STZ) or with the citrate buffer vehicle (CTL). A–D. Retinal flatmounts and sections of P6 CTL (A–B) and STZ (C–D) were co-stained using anti-collagen IV antibody and anti-GFAP antibody. E–F Retinal flatmounts of P6 CTL (E) and STZ (F) were co-stained using anti-collagen IV antibody and or anti-Ng2 antibody. G–H. STZ animals with hyperglycemia >400 mg/DL were co-stained using anti-collagen IV antibody and anti-Ng2 antibody. Nuclei were counterstained with DAPI in A and C. Scale bar 50 µm in A–G, g and g′; 5 µm in E and F insets and H.