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Cell lineage studies. A. Co-expression of AFP and insulin in E14-initiated culture transduced with Ad-PNM. B-C. Cultures from Sox9CreER embryos were treated with tamoxifen and subsequently with Ad-PNM. Sox9 expressing cells become and remain green and some insulin-positive cells are green (examples indicated with arrowheads). Insulin is viewed in far red, rendered as magenta. Scale bars 100 m. D. Quantification of results. This experiment used 4d tamoxifen treatment.
