Table II.
Association between log transformed Cyp19 granulosa cell gene expression and urinary specific gravity adjusted BPA quartiles among 61 women (76 IVF cycles) based on mixed effects models, accounting for the correlation between outcomes.
| Covariates | Unadjusted | Adjusteda | Batch- adjustedb | |||
|---|---|---|---|---|---|---|
|
| ||||||
| Parameter estimate | Parameter estimate | Parameter estimate | ||||
| (95% CI) | P-value | (95% CI) | P-value | (95% CI) | P-value | |
| Log SG-adjusted BPA | 0.12(−0.46, 0.69) | 0.67 | 0.06 (−0.53, 0.64) | 0.84 | 0.001 (−0.54, 0.54) | 1.00 |
|
| ||||||
| BPA quartiles (range ng/L) | ||||||
| Q1 (≤1.32) | ref | ref | ref | ref | ref | ref |
| Q2 (1.32-1.98) | −0.64(−1.94, 0.66) | 0.30 | −0.74(−2.09, 0.62) | 0.26 | −0.97 (-2.22, 0.28) | 0.11 |
| Q3 (1.98-3.27) | −0.53-1.81, 0.75) | 0.38 | −0.69 (−2.10, 0.62) | 0.27 | −0.97 (-2.18, 0.24) | 0.10 |
| Q4 (≥3.27) | −0.13(−1.41, 1.15) | 0.83 | −0.29 (−1.60, 1.03) | 0.64 | −0.38 (−1.58, 0.82) | 0.49 |
|
| ||||||
| Test for trend (p-value)c | 0.90 | 0.69 | 0.56 | |||
|
| ||||||
| BMI (continuous) | −0.06(−0.15, 0.04) | 0.21 | −0.07 (−0.17, 0.03) | 0.16 | −0.09 (−0.18, 0.01) | 0.06 |
| Day 3 FSH IU/L)* | 0.05(−0.20, 0.30) | 0.69 | --- | --- | --- | --- |
| IVF protocol (flare/ant. vs. luteal) | −0.04(−0.96, 0.88) | 0.93 | --- | --- | --- | --- |
| Age (years) | −0.05(−0.15, 0.05) | 0.35 | --- | --- | --- | --- |
| Batch (year 2012 vs year 2011) | −1.54(−2.47, -0.61) | 0.003 | --- | --- | −1.76 (−2.73, −0.80) | 0.002 |
Abbreviations: BPA, Bisphenol A; BMI, body mass index; Day 3 FSH, follicle stimulating hormone on 3rd day of menses; 95% CI=95% confidence intervals; batch = year of shipment
*
missing day 3 FSH on 17 women, therefore this covariate was excluded from multivariate analyses
a
adjusted for BMI;
b
adjusted for BMI and batch effect (year 2012 versus year 2011);
c
test for linear trend based on ordinal quartiles