Figure 4. Change in number of CD4⁺T-cell lymphocytes in EAC mice after anti-CXCL10 Ab treatment.

At the end of experiments, lymphocytes from the spleen, iliac lymph nodes, and urinary bladder were isolated, stained for CD4⁺ T cell expression, and analyzed by flow cytometry. A representative histogram is shown with the mean percentage of spleen, iliac lymph node, and urinary bladder CD4⁺ cells per mouse. The data presented are representative dot plots and histograms from three independent experiments involving 5 mice per group.