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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Cell Biochem Biophys. 2014 Jan;68(1):10.1007/s12013-013-9677-6. doi: 10.1007/s12013-013-9677-6

Figure 3. Inhibition of Ca2+-induced muscle contraction by activators of PKG independent of RhoA.

Figure 3

(A) Permenabilized muscle cells were treated with Ca2+ (10 μM) or aceycholine (ACh, 1 μM) for 10 min and Rho kinase activity was measured by immunokinase assay as described in the methods. Results are expressed as cpm/mg protein. Values are means ± SE of 4 experiments. **P < 0.01, significant increase in Rho kinase activity. (B) Permeabilized muscle cells were treated with various concentrations of GSNO or cGMP in the presence of Rho kinase inhibitor Y27632 (10 μM) for 10min, and then stimulated with 10 μM Ca2+ for 30 s to induce muscle contraction. In control experiments the cells were treated for 30 s with Ca2+ in the presence of Y27632 without prior addition of GSNO or cGMP. Relaxation was expressed as % inhibition of Ca2+-induced contraction (31.3±2.8 μm decrease from control cell length of 96.2±4.3 μm). Values are means ± SE of 4–5 experiments.