Assessment of Class of Evidence (CoE) for individual studies of diagnostic test evaluation.

Methodological principle
Study design
Prospective cohort design	X
Retrospective cohort design
Case–control design
Broad spectrum of patients with expected condition	a
Appropriate reference standard used	X
Adequate description of test and reference for replication	X
Blinded comparison with appropriate reference	X
Reference standard performed independently of test	X
Evidence level	II
Note: Blank box indicates criterion not met, could not be determined, or information not reported by author or was not reported. aThis study contained nine animal subjects.