Table 1. Primers of quantitative RT-PCR analysis of candidate defence genes. -f represents forward primers and -r represents reverse primers.
Potential gene function | Size (bp) | Primer | Sequence |
purple acid phosphatase 23 | 1224 | m. 16215-f | GCCTGAATCAATGTCAATACC |
m. 16215-r | CTGCTGCTACTACAATTATGC | ||
WRKY transcription factor 20 | 1098 | m. 28831-f | GCAGGTTCTTGACATCATTAG |
m. 28831-r | AGGAAGAACAGGAACAAGAGAAC | ||
hexose carrier | 696 | m. 34711-f | GTCTCATTCTATGCTCCACTATTG |
m. 34711-r | ACCCTTCTGCCTACTTTATCTAC | ||
heat shock protein 70 | 1224 | m. 35228-f | CCACCAGCGGACAAGAAG |
m. 35228-r | GCCATATCAGCACCACCAG | ||
glutathione S-transferase | 438 | m. 31344-f | TGGCTGGATTGGAATTACTG |
m. 31344-r | CGTACATGGTCGGATAAGG | ||
pyruvate kinase | 1398 | m. 50337-f | CAGTATGGCTTGAGGTTATAG |
m. 50337-r | AAGGAAGTCTATGTTGTTGC |