Figure 3. Anti-donor T cell responses are regulated through the cholinergic anti-inflammatory pathway.

(A to C): Splenocytes harvested from either α7+/+ (white bars) or α7−/− (black bars) male to female donor-recipient combinations were stimulated in mixed lymphocyte reactions with α7nAchR-matched female or male splenocytes. IL-2 (A), IL-17 (B) and IFN-γ (C) production in the supernatants was quantified by ELISA. (F) indicates female and (M) indicates male. (*): p<0.05 compared with wild type α7+/+ donor-recipient combination or female stimulation.