Skip to main content
. 2013 Nov 21;7(11):e2564. doi: 10.1371/journal.pntd.0002564

Figure 1. DHFR-TS amplification and resistance to 5-fluorouracil.

Figure 1

(A). Comparative genomic hybridization (CGH) experiments between wild-type and Lin5FU500.2 cells. Grey, equal amount of DNA between the two strains; black, increased copy number of DNA in the mutant Lin5FU500.2. (B). Southern blots of total digested DNAs isolated from WT and each mutant strains at 0 and 30 passages without drug were hybridized to a specific DHFR-TS probe. The DNA was digested to discriminate the chromosomal copy (C*) from the amplified copy (A*). (C). Role of DHFR-TS in 5-FU resistance. Growth curves of wild-type L. infantum parasites transfected with the expression construct pSP72αHYGα- DHFR-TS (black squares) or with the empty vector pSP72αHYGα (white squares) or the Lin5FU500.2 revertant transfected with pSP72αHYGα- DHFR-TS (black circles) or pSP72αHYGα (white circles). Average of at least three independent experiments. Transfection of DHFR-TS led to 5FU resistance that was statistically significant compared to mock transfectants (p<0.05).