Table 1. Resistance levels to 5-FU and MTX in L. infantum 263 WT and 5-FU resistant mutants.

	EC50 (µM)
	5FU	MTX	5FU Rev*
L. infantum 263 WT	72±0.8	114±9	-
L. infantum 5FU500.1	>2000	207±2**	927±83
L. infantum 5FU500.2	>2000	345±5**	78±15
L. infantum 5FU500.3	>2000	5±0.3**	>2000
L. infantum 5FU500.4	>2000	106±4	>2000
L. infantum 5FU500.5	>2000	96±3	>2000

^*Rev = revertant strain cultured without drug pressure over 30 passages;

^**(p<0,005).