Table 2. Whole genome SNP discovery in 5-FU resistant mutants.
| Strain | Chr | Base in GeneDB | Base in mutant | Gene ID | Gene products | Position in the gene | a.a. change | Individual Sequencing* |
| Lin5FU500.1 | 14 | C | G | LinJ.14.1190 | kinesin K39 | 4557 | H1519Q | ND |
| 15 | T | C | LinJ.15.0490 | hypothetical protein | 8693 | L2898S | ND | |
| 33 | G | T | LinJ.33.2730 | hypothetical protein | 205 | G69C | ND | |
| 35 | A | C | LinJ.35.0500 | proteophosphoglycan ppg3 | 12698 | E4233A | ND | |
| 36 | G | T | LinJ.36.1020 | hypothetical protein | 261 | S87S | ND | |
| Lin5FU500.2 | 3 | C | T | LinJ.03.0260 | hypothetical protein | 3902 | A1301V | ND |
| 3 | C | T | LinJ.03.0260 | hypothetical protein | 4136 | A1379V | ND | |
| 10 | A | C | LinJ.10.1030 | eIF-2B GDP-GTP exchange factor | 1298 | V433G | ND | |
| 15 | A | G | LinJ.15.0490 | hypothetical protein | 2811 | L937L | ND | |
| 35 | T | G | LinJ.35.0490 | proteophosphoglycan ppg4 | 7888 | S2630A | ND | |
| 35 | A | G | LinJ.35.0490 | proteophosphoglycan ppg4 | 8751 | S2917S | ND | |
| 35 | T | G | LinJ.35.4860 | AMP deaminase | 2730 | A910A | ND | |
| Lin5FU500.3 | 6 | C | G | LinJ.06.1360 | hypothetical protein | 1744 | P582A | E |
| 14 | C | G | LinJ.14.0790 | fatty acid elongase | 471 | M157I | ND | |
| 16 | A | G | LinJ.16.1030 | hypothetical protein | 2704 | R902G | ND | |
| 21 | T | G | LinJ.21.1450 | thymidine kinase | 260 | Q87P | M | |
| 35 | T | G | LinJ.35.0520 | proteophosphoglycan ppg4 | 7366 | F2456V | ND | |
| 35 | C | A | LinJ.35.4450 | hypothetical protein | 994 | Q332K | ND | |
| Lin5FU500.4 | 28 | T | C | LinJ.28.2390 | cyclin dependent kinase-binding protein | 1301 | L434P | ND |
| 29 | A | C | LinJ.29.2100 | hypothetical protein | 2673 | T891T | ND | |
| 34 | A | C | LinJ.34.0820 | serine/threonine phosphatase PP1 | 773 | E258A | E | |
| 34 | A | C | LinJ.34.0830 | serine/threonine phosphatase PP1 | 836 | E279A | E | |
| 34 | A | C | LinJ.34.1110 | uracil phosphoribosyl transferase | 434 | K145T | M | |
| 34 | A | C | LinJ.34.2220 | hypothetical protein | 5335 | S1779A | ND | |
| 34 | C | G | LinJ.34.3040 | hypothetical protein | 1907 | S636W | M | |
| Lin5FU500.5 | 10 | A | C | LinJ.10.1090 | uridine phosphorylase | 794 | L265R | M |
| 20 | G | C | LinJ.20.0750 | hypothetical protein | 3132 | Q1044H | ND | |
| 29 | A | G | LinJ.29.1890 | paraflagellar rod protein 1D | 801 | D267D | ND | |
| 35 | A | G | LinJ.35.0490 | proteophosphoglycan ppg4 | 6723 | A2241A | ND |
*
Experimental validation of SNP variants was performed using PCR-directed sequencing using appropriate pairs of primers (see Table S1, Supplementary Material). Following SNP validation, the WT version of each mutated gene indicated in bold were transfected in the L. infantum 263 WT, Lin5FU500.3, Lin5FU500.4 and Lin5FU500.5 strains. SNPs in italic indicate silent mutations. M, mutation; E, sequencing error; ND, not determined.