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. 2013 Nov 16;62(12):4030–4042. doi: 10.2337/db13-0627

FIG. 3.

FIG. 3.

Effects of SNP on FITC-insulin uptake by ECs pretreated with inhibitors of insulin action. bAECs were serum starved for 6 h then pretreated with either 100 nmol/L wortmannin (WOT) or 10 μmol/L PP1 for 30 min followed by 50 nmol/L FITC-insulin ± 0.3 μmol/L SNP or vehicle (VEC) for 30 min before fixation and immunocytochemical staining. A: Representative confocal images of bAECs stained for FITC from three independent experiments. B and C: Quantitative analysis of cellular insulin uptake for each experimental condition. #, *, and **P < 0.05 compared with remaining groups (B); * and #P < 0.05 compared with remain groups (C). D: Representative confocal images of bAECs that were serum starved ± 5 ng/mL TNF-α for 6 h followed by 50 nmol/L FITC-insulin ± 0.3 μmol/L SNP or VEC for 30 min before fixation and immunocytochemical staining from three independent experiments. E: Quantitative analysis of the cellular insulin uptake presented in D. * and #P < 0.05 compared with remaining groups and with each other.