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. 2013 Nov 16;62(12):4030–4042. doi: 10.2337/db13-0627

FIG. 4.

FIG. 4.

Effects of cGMP analog and ODQ on insulin uptake. bAECs were serum starved for 6 h then pretreated with either 8-bromo-cGMP (0.01, 0.1, 2, or 4 mmol/L) for 5 min or ODQ (2 or 20 μmol/L or vehicle) for 15 min followed by FITC-insulin 50 nmol/L ± 0.3 μmol/L SNP for 30 min before fixation and immunocytochemical staining. A: Representative confocal images of bAECs stained for FITC from three independent experiments. B: The histograms indicate the dose response of FITC-insulin uptake to 8-bromo-cGMP treatment. *P < 0.01 compared with the remaining groups. C: Quantitative analysis of cellular insulin uptake for each experimental condition. * and **P < 0.05 compared with remaining groups. cGMP10, cGMP 0.01 mmol/L; cGMP100, cGMP 0.1 mmol/L; INS, insulin; ODQ2, ODQ 2 μmol/L; ODQ20, ODQ 20 μmol/L; VEC, vehicle.