FIG. 5.

Cytokine expression in adipocytes and SVF, and immune composition of SVF from STAT4^−/−C57Bl6 and control mice on chow diet. Gene expression of proinflammatory cytokines in adipocytes (A) and different Th2 markers in SVF (B) of knockout and control chow-fed mice 26–28 weeks of age. Data are expressed as the fold change in STAT4^−/−C57Bl6 expression compared with control after normalization to β-actin. Data are the average ± SEM from n = 6–8 mice per group. Relative composition of different T-cell subsets (C) and granulocytes (D) in SVF of STAT4^−/−C57Bl6 and C57Bl6 controls on chow diet. In both cases, cells were analyzed in the positive CD45 gate. Data are expressed as the relative percentage of cells and represent the mean ± SEM from n = 6–8 mice per group. *Significant difference for P < 0.05.