FIG. 7.

Metabolic phenotype and immune cell composition in AT of Rag1-null mice reconstituted with splenocytes from STAT4-deficient mice and C57Bl6 controls. Eight-week-old Rag1-null mice were injected with splenocytes from either STAT4-deficient mice or wild-type controls and placed on HFD for 15 weeks. A: Fasted plasma glucose and insulin levels, HOMA-IR, body weight, and food intake were measured along with intraperitoneal ITTs and GTTs. AUCs were calculated, and the results represent the mean ± SEM from n = 9–10 mice per group. B: SVF was isolated from AT, and the cells were fluorescently labeled with CD45, CD3, CD8, CD206, and F40/80 anti-mouse antibodies. Cells were gated for CD45 positivity and selected for viability using propidium iodide (PI) exclusion. Representative plots from each group for CD3⁺CD8⁺ cells and for CD206⁺F4/80⁺ cells are shown in the bottom plots. Results represent the mean ± SEM from 4–5 mice per group. n.s., nonsignificant.