FIG. 3.

Localization of ADORA1 in human islets. A: Confocal microscopy images show colocalization of ADORA1 with glucagon in most of the ADORA1-positive cells of nondiabetic control patients. Some ADORA1-positive cells did not colocalize with glucagon (arrows). The islets of AA+ individuals expressed less ADORA1 than controls (B), and long-term T1D patients had little or no ADORA1 staining (C). D: ADORA1 was also found to colocalize with insulin in the islet of a control subject (arrow). nPOD patient information is reported in Table 1. Data are representative of ≥10 similarly stained islets. Scale bars represent 50 μm.