FIGURE 2.

E2 inhibits thapsigargin-induced STIM1 translocation in HBECs. A, typical XZ confocal micrographs of STIM1 immunocytochemistry (red) with actin counterstain (phalloidin, green). Representative images were from one of several experiments. The cells were either untreated (Basal), stimulated with thapsigargin (2 μm) to deplete ER Ca²⁺, with E2 (10 nm), or with E2 and thapsigargin. B, graph of mean pixel intensity of STIM1 in relationship to the apical membrane. Data are shown as mean ± S.E. Scale bar represents 25 μm.