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. 2013 Oct 10;288(47):33509–33518. doi: 10.1074/jbc.M113.486662

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — E2 inhibits STIM1 aggregation and redistribution. A, typical images of STIM1-mCherry (red) and ESR1-CFP (cyan) in HEK293T cells under control or E2 pretreatment conditions ± TG. B, percentage of HEK293T cells containing STIM1 puncta. Ctrl, basal n = 46, TG n = 47; E2, basal n = 45, TG n = 48. C, typical confocal micrographs of Orai1-YFP (green, donor) and STIM1-mCherry (red, acceptor) before and after photobleaching of STIM1-mCherry in control cells under resting conditions. Cells also expressed ESR1-CFP (data not shown). D, mean STIM1-Orai1 FRET efficiency (%E) in HEK293T cells. Ctrl, basal n = 39, TG n = 31; E2, basal n = 39, TG n = 33. *, significant difference (one-way ANOVA followed by Tukey's multiple comparison test; p < 0.001). Data are shown as mean ± S.E. Scale bars represent 10 μm.