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. 2013 Oct 10;288(47):33509–33518. doi: 10.1074/jbc.M113.486662

FIGURE 5.

FIGURE 5.

E2 inhibits STIM1 mobility. A, typical images of YFP-STIM1 in HEK293T cells before and after photobleaching (ESR1-CFP not shown). Dashed circles indicate the area that was bleached. B, normalized recovery of YFP-STIM1 after photobleaching in HEK293T cells co-expressing ESR1-CFP under basal conditions (n = 15) and after thapsigargin stimulation (n = 13) or when pretreated with E2 (n = 17) and after E2 with thapsigargin (n = 16). C, mobile fraction of YFP-STIM1 in HEK293T cells co-expressing ESR1 under each condition. D, normalized recovery of YFP-STIM1 after photobleaching in HEK293T under basal conditions (n = 13) and after thapsigargin stimulation (n = 10) or when pretreated with E2 (n = 9) and after thapsigargin stimulation (n = 10). E, mobile fraction of YFP-STIM1 in HEK293T cells under each condition. *, significant difference of p < 0.05; **, significant difference of p < 0.001 (one-way ANOVA followed by Tukey's multiple comparison test). Data are shown as mean ± S.E. Scale bar represents 10 μm.