FIGURE 1.

Thrombin-induced phosphorylation of CaMKII and CREB. A, VSM cells were treated with thrombin (50 nm) or ionomycin (Iono; 0. 5 μm). Cell lysates were resolved by SDS-PAGE and immunoblotted for phospho-Thr²⁸⁷-CaMKII (pThr287), total CaMKIIδ, phospho-Ser¹³³-CREB (pSer133), phospho-Ser¹⁴²-CREB (pSer142), and β-actin. B, ECL signals were quantified with a FujiLAS 4000. The plot quantifies CaMKII-p287 as an index of CaMKII activation, CREB-Ser(P)¹³³ and CREB-Ser(P)¹⁴². Data were normalized for loading using β-actin signals and expressed as fold-values compared with unstimulated. Values shown are mean ± S.E., n = 3 and were analyzed by one-way ANOVA. *, p < 0.05 and **, p < 0.01, by Dunnett's Multiple comparison post hoc test.