FIGURE 4.

Inhibition of CaMKII activity blocks CREB phosphorylation on Ser¹⁴². A, VSM cells were pretreated with KN93 (30 μm) for 30 min and stimulated with thrombin (50 nm) or ionomycin (0.5 μm) for 1 min. Cell lysates were resolved by SDS-PAGE and immunoblotted for phospho-Thr²⁸⁷-CaMKII (pThr287), phospho-Ser¹⁴²-CREB (pSer142), and GAPDH. B, quantification of CaMKII-Thr(P)²⁸⁷ ECL signals. C, quantification of CREB-Ser(P)¹⁴² ECL signals. Data were normalized for loading using GAPDH signals and expressed as fold-values compared with unstimulated. Values shown are mean ± S.E. n = 3 and analyzed by unpaired two-tailed Student's t test. **, p < 0.01.