FIGURE 7.
CaMKIIδ inhibits CREB reporter activity and CREB-regulated genes. A and B, CREB luciferase reporter stimulated by thrombin (50 nm) or forskolin (5 μm) for 6 h. Adenovirus encoding a dominant-negative CaMKIIδ2 mutant (DN-CaMKII) (A) or constitutively active CaMKIIδ2 mutant (CA-CaMKII) (B) was transduced for 48 and 24 h prior to cell lysis, respectively (see insets for immunoblots (IB) of CaMKIIδ expression). The plotted values are expressed as fold of unstimulated cells transduced with an adenovirus control (Ad-virus). C, mRNA expression of Sik1 (n = 5), and D, Rgs2 (n = 4) was determined by RT-PCR in cells treated as described above. Ct values were normalized to GAPDH. Changes in mRNA in each group are fold-values of the unstimulated control. Values shown are mean ± S.E. and analyzed by one-way ANOVA. *, p < 0.05; **, p < 0.01, by Bonferroni post hoc test.