FIGURE 4.

Phd2^P294R/+ mice display erythrocytosis. A–D, hematocrit (A, HCT), hemoglobin (B, Hb), red blood cell counts (C, RBC), and white blood cell counts (D, WBC) were measured in mice bearing the indicated Phd2 alleles (age = 12–13 months; n = 8/group). *, p < 0.05 relative to WT controls by ANOVA with pairwise Tukey HSD. E, hematocrit measurements from mice bearing the indicated Phd2, Hif1a, and Hif2a alleles (n = 5/group). *, p < 0.05 relative to WT controls by ANOVA with pairwise Tukey HSD. F, Western blots (WB) examining levels of the indicated proteins in kidney extracts from wild type, Phd2^P294R/+, Phd2^+/−, Hif1a^+/−, and Hif2a^+/− animals.