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AtAMY3 and AtBAM1 degrade starch synergistically in vitro. Recombinant proteins AtAMY3 (10 μg) and/or AtBAM1 (2.5 μg) were incubated at 25 °C with purified Arabidopsis starch (1.5 mg). After the indicated time points, soluble glucans in the supernatant were removed and subjected to acid hydrolysis. Glucose was subsequently quantified. Values represent the mean ± S.E. (n = 3). Error bars not visible are smaller than the symbols.
