TABLE 1.

Quantification of peak areas from Fig. 2A

Values represent relative peak areas from HPAEC-PAD chromatograms of malto-oligosaccharides released from Arabidopsis starch granules by AtAMY3 or HvAMY1. Starch (1.5 mg) was digested with AtAMY3 (10 μg) or HvAMY1 (50 ng) for 30 or 120 min, and the glucans released were analyzed. A representative chromatogram is presented in Fig. 2A. The relative peak area was calculated for each peak as a percentage of total peak area. Values represent the mean ± S.E. from chromatograms produced from three replicate digests.

	DP	AtAMY3		HvAMY1
		30 min	120 min	30 min	120 min
Linear glucans	3	4.00 ± 0.09	5.71 ± 0.03	2.78 ± 0.05	4.28 ± 0.03
	4	9.8 ± 0.1	12.28 ± 0.04	2.878 ± 0.008	3.78 ± 0.03
	5	7.00 ± 0.03	7.96 ± 0.01	1.191 ± 0.006	1.87 ± 0.02
	6	13.60 ± 0.06	16.07 ± 0.05	10.38 ± 0.02	13.0 ± 0.1
	7	22.76 ± 0.05	23.68 ± 0.01	34.1 ± 0.2	36.6 ± 0.3
	8	16.74 ± 0.05	13.70 ± 0.05	15.5 ± 0.2	13.66 ± 0.03
	9	10.19 ± 0.07	5.49 ± 0.06	7.96 ± 0.08	6.06 ± 0.01
	10	4.47 ± 0.07	1.09 ± 0.03	6.13 ± 0.06	4.33 ± 0.02
	11	1.03 ± 0.03	0.162 ± 0.008	4.42 ± 0.05	2.96 ± 0.01
	12	0.293 ± 0.007	0.096 ± 0.004	3.42 ± 0.04	2.22 ± 0.01
	13	0.186 ± 0.003	0.133 ± 0.001	2.60 ± 0.03	1.669 ± 0.007
	14	0.137 ± 0.002	0.162 ± 0.002	1.92 ± 0.03	1.245 ± 0.007
	15	0.119 ± 0.002	0.248 ± 0.002	1.31 ± 0.02	0.824 ± 0.002
Branched glucans	8	0.08 ± 0.04	0.295 ± 0.002	0.009 ± 0.009	0.07 ± 0.04
	9	0.31 ± 0.02	0.82 ± 0.01	0.303 ± 0.003	0.546 ± 0.006
	10	0.838 ± 0.007	1.64 ± 0.01	0.639 ± 0.004	0.942 ± 0.007
	11	1.30 ± 0.01	2.19 ± 0.02	0.537 ± 0.004	0.881 ± 0.007
	12	1.539 ± 0.004	2.17 ± 0.01	0.550 ± 0.005	0.91 ± 0.01
	13	1.596 ± 0.004	1.965 ± 0.007	0.622 ± 0.003	0.96 ± 0.01
	14	1.534 ± 0.005	1.66 ± 0.001	0.635 ± 0.005	0.941 ± 0.009
	15	1.345 ± 0.008	1.275 ± 0.002	0.545 ± 0.002	0.763 ± 0.004