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AtAMY3 peptides confirmed by MALDI-MS/MS
Peptides generated after in-solution digest with LysC were visualized by MALDI-MS (Fig. 8), and the corresponding MS/MS spectra were searched with Mascot against the recombinant protein sequence. Mascot scores are from spectra generated with the reduced wild type protein.
| m/z | Peptide | Score |
|---|---|---|
| 1679.9 | VLGDAVLNHRC*AHFK (* is Cys587) | 70 |
| 2361.1 | ISSGTGSGFEILC*QGFNWESNK (* is Cys499) | 91 |
