FIGURE 2.

Suppression of p62 attenuates drug-induced apoptosis. A and B, HCT116 cells with stable expression of lentiviral p62 shRNA or control (ctrl) were incubated with ABT-263 (48 h) (A) or CPT-11 (5 days) (B), and apoptosis was quantified by annexin V+ staining using flow cytometry. The mean values of triplicate experiments are shown; bars represent S.D. C and D, HCT116 (C) or DLD1 (D) cells with suppression of p62 by shRNA versus control were incubated with ABT-263 at the indicated doses for 48 h, and expression of p62 and caspase cleavage were analyzed by immunoblotting. E, effect of p62 suppression by shRNA on ABT-263-induced caspase-3 cleavage.