FIGURE 2.
Specificity of the anti-hα1A antibody. A–C, representative images of SY5Y cells transfected with EGFPα1AWT/β3/α2δ or EGFP-α1AE1015K/β3/α2δ subunits and immunostained with affinity-purified anti-hα1A antibody before (A and C) or after preincubation (B) with the peptide used for immunization (preadsorbed). D, representative Western blots of total cell extracts (40 μg of proteins) from HEK293 cells transfected with WT channels and labeled with anti-hα1A or anti-hα1A antibodies preincubated with antigen.