FIGURE 7.
SNARE proteins do not modulate E1015K CaV2.1 channel function. A, representative Western blots of cell lysates (30 μg of total proteins) obtained from HEK cells co-expressing syntaxin 1A or SNAP-25 with either WT or E1015K CaV2.1 and probed with antibodies against human α1A, β3, syntaxin 1A, or SNAP-25. B, representative current traces from cells expressing WT or E1015K CaV2.1 channels in the presence or absence of syntaxin 1A. C and D, effect of syntaxin 1A or SNAP-25 expression on steady-state inactivation curves recorded from WT (C) or E1015K (D) CaV2.1-expressing cells (n = 7–9). E, syntaxin 1A and SNAP-25 significantly decreased the Vhalf of inactivation of WT channels (where ** indicates p < 0.01) but had no effect on E1015K channels. Error bars, S.E.