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. 2013 Oct 9;288(47):33873–33883. doi: 10.1074/jbc.M113.497701

FIGURE 7.

FIGURE 7.

SNARE proteins do not modulate E1015K CaV2.1 channel function. A, representative Western blots of cell lysates (30 μg of total proteins) obtained from HEK cells co-expressing syntaxin 1A or SNAP-25 with either WT or E1015K CaV2.1 and probed with antibodies against human α1A, β3, syntaxin 1A, or SNAP-25. B, representative current traces from cells expressing WT or E1015K CaV2.1 channels in the presence or absence of syntaxin 1A. C and D, effect of syntaxin 1A or SNAP-25 expression on steady-state inactivation curves recorded from WT (C) or E1015K (D) CaV2.1-expressing cells (n = 7–9). E, syntaxin 1A and SNAP-25 significantly decreased the Vhalf of inactivation of WT channels (where ** indicates p < 0.01) but had no effect on E1015K channels. Error bars, S.E.