FIGURE 14.

N-Glycosylation is important for membrane localization of Oatp1d1. Tunicamycin, a general inhibitor of N-glycosylation, reduced E3S (A) and LY (B) uptake in a dose-dependent manner and partly impaired membrane localization of Oatp1d1 as shown by immunofluorescence analysis (inset). Immunocytochemistry was performed after a 24-h exposure to tunicamycin (5 μg/ml), with FITC antibody (green) and nuclei stained with DAPI (blue). Uptake is expressed as a percentage relative to tunicamycin-non-treated Oatp1d1-transiently transfected HEK293 cells. Results represent means and S.E. (error bars) of three independent experiments.