FIGURE 6.
Full-length NELF-B and NELF-A act at two steps in competition assays with GREtkLUC and with GR. U2OS cells were transiently transfected with the indicated varying amounts of GREtkLUC reporter, NELF-B, or NELF-A plasmid or GR plasmid as described under “Experimental Procedures.” The Amax and EC50 were determined as described for Fig. 5D. Plots, after correcting for nonlinear expression of chimeric NELF-B as described under “Experimental Procedures,” of 1/EC50 versus GREtkLUC for NELF-B (A), EC50/100 × Amax versus NELF-B (B, D, and F) or versus NELF-A (H), and Amax/100 × EC50 versus GR with NELF-B (C and E) and with NELF-A (G) were made and interpreted as described under “Results.” Similar results were seen in 3 or 4 additional independent experiments.