Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Oct 9;288(47):34168–34180. doi: 10.1074/jbc.M113.484493

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 6. — Single-molecule FRET assay for RAD51-ssDNA filament formation. A, illustration of the DNA FRET substrates used in our assays, consisting of partial duplex DNA with a dT30 or dT20 tail. B, illustration of single-molecule RAD51-ssDNA filament assembly assays. The DNA substrate shown on the left yields high FRET at steady state. Upon the addition of RAD51 and ATP, a RAD51-ssDNA filament forms, extending the ssDNA, resulting in a decrease in FRET (right). C and D, FRET histograms of a population of individual DNA molecules for the 20-nt (C) and 30-nt (D) substrates, showing the resulting FRET values when ssDNA is relaxed (DNA only; blue histogram) and after the assembly of a RAD51K133R-ssDNA filament, resulting in a FRET decrease (gray). E, representative smFRET trajectory of a 30-nt DNA substrate with a RAD51K133R-ssDNA filament. The top panel shows the donor and acceptor intensities, whereas the corresponding FRET efficiency is shown in the bottom panel. The FRET efficiency associated with stable RAD51K133R-ssDNA is marked with a horizontal dashed line.