FIGURE 5.

CRP-induced RhoG activation and its inhibition by PP2. A, RhoG activation was measured upon stimulation of washed human platelets with 5 μg/ml CRP for various times. Washed platelets were lysed, and active GTP-bound RhoG was determined by pulldown analysis using bacterially expressed GST-ELMO. B, RhoG activation induced by 5 μg/ml CRP for 60 s was evaluated in the presence and absence of 10 μm PP2, 2 μm OXSI-2, or 100 nm wortmannin (Wort). The blot shown is representative of three independent experiments.