FIGURE 6.

Fucoidan-induced platelet responses are not affected by RhoG deficiency. A, washed platelets from RhoG^−/− mice and RhoG^+/+ littermates were stimulated with 100 μg/ml fucoidan (a selective CLEC-2 agonist), and platelet aggregation was measured by aggregometry. B, wild-type and RhoG-deficient platelets were stimulated with 100 μg/ml fucoidan and probed with anti-phospho (P)-Syk (Tyr⁵²⁵/Tyr⁵²⁶), anti-phospho-Akt (Ser⁴⁷³), and anti-β-actin (lane loading control) antibodies by Western blotting. Data are representative of three independent experiments. Cont, control; KO, knock-out.