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Association/dissociation of MJ and MJI homodimers followed by changes in LRET. A, MJ. MJ concentration was 1 μm. Half of the MJ was labeled with Tb3+ and the other half with fluorescein. Effects of successive addition of 0.5 mm ATP, 10 mm MgCl2, and 10 μm unlabeled MJ (arrow) are shown. The signal was normalized to the ATP-induced change. B, MJI. The experiment was similar to that in A, but used 1 μm MJI.
