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. 2013 Oct 30;10(12):1795–1804. doi: 10.7150/ijms.7025

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Cell proliferation after down-regulation or over-expression of GINS2 in HL60 cells respectively. A, Down-regulation of GINS2 expression showed that colony-forming activities in psiGINS2-2 group decreased significantly on soft agar through counting numbers of colonies (1,psiGINS2-2, 2,NC siRNA and 3,untreated control). B, The colonies of psiGINS2-2, NC siRNA and untreated control group formed on soft agar. C, MTT assay was performed to measure proliferation potential of cells after silencing of GINS2. D, UP-regulation of GINS2 expression showed that colony-forming activities in pAd-GINS2 group increased remarkably on soft agar through counting numbers of colonies (1,pAd-GINS2, 2,pAd-KZ and 3, untreated control). E, The colonies of pAd-GINS2, pAd-KZ and untreated control group formed on soft agar. F, MTT method was executed to detect cell proliferation after over-expression of GINS2. Each assay condition was carried out in triplicate in at least two independent batches. Data was represented as means ± SD of three impendent experiments. *P <0.05 (vs. control).

Cell proliferation after down-regulation or over-expression of GINS2 in HL60 cells respectively. A, Down-regulation of GINS2 expression showed that colony-forming activities in psiGINS2-2 group decreased significantly on soft agar through counting numbers of colonies (1,psiGINS2-2, 2,NC siRNA and 3,untreated control). B, The colonies of psiGINS2-2, NC siRNA and untreated control group formed on soft agar. C, MTT assay was performed to measure proliferation potential of cells after silencing of GINS2. D, UP-regulation of GINS2 expression showed that colony-forming activities in pAd-GINS2 group increased remarkably on soft agar through counting numbers of colonies (1,pAd-GINS2, 2,pAd-KZ and 3, untreated control). E, The colonies of pAd-GINS2, pAd-KZ and untreated control group formed on soft agar. F, MTT method was executed to detect cell proliferation after over-expression of GINS2. Each assay condition was carried out in triplicate in at least two independent batches. Data was represented as means ± SD of three impendent experiments. *P <0.05 (vs. control).